HEPES is a zwitterionic N-substituted acidic buffering substance used for culturing cells in vitro. HEPES is also useful in transfections of mammalian cell lines.
| M | 238.31 |
|---|---|
| m.p | 52 - 58 °C |
| Appearance | White to off-white powder |
|---|---|
| Iron (Fe) | max. 0.0005% |
| Loss on drying (105°C) | max. 0.5% |
| Potassium (K) | max. 0.1% |
| Heavy metals (as Pb) | max. 0.001% |
| pH (0.5M in water) | 5-6.5 |
| Sulfate (SO4) | max. 0.05% |
| Assay (T, dry) | 99.5-101.0%w/w |
| A260nm (0.5M) | max. 0.05AU |
| A280nm (0.5M) | max. 0.05AU |
| DNase activity | Not detected |
| RNase activity | Not detected |
| Protease activity | Not detected |
| Synonym : 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) |
| Formula : C8H18N2O4S |
| CAS : 7365-45-9 |
| EC : 230-907-9 |
| 08042381 | 1 x 20kg | Drum (20kg) |
| 08042391 | 6 x 1kg | HDPE (1kg) |
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