HEPES free acid Molecular biology

HEPES is a zwitterionic N-substituted acidic buffering substance used for culturing cells in vitro. HEPES is also useful in transfections of mammalian cell lines.

Main characteristics

m.p52 - 58 °C

Product specificities

AppearanceWhite to off-white powder
Iron (Fe)max. 0.0005%
Loss on drying (105°C)max. 0.5%
Potassium (K)max. 0.1%
Heavy metals (as Pb)max. 0.001%
pH (0.5M in water)5-6.5
Sulfate (SO4)max. 0.05%
Assay (T, dry)99.5-101.0%w/w
A260nm (0.5M)max. 0.05AU
A280nm (0.5M)max. 0.05AU
DNase activityNot detected
RNase activityNot detected
Protease activityNot detected

Cat. n° 080423

Synonym : 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
Formula : C8H18N2O4S
CAS : 7365-45-9
EC : 230-907-9

Standard pack

080423811 x 20kgDrum (20kg)
080423916 x 1kgHDPE (1kg)

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