C8H18N2O4S
HEPES free acid Molecular biology
HEPES is a zwitterionic N-substituted acidic buffering substance used for culturing cells in vitro. HEPES is also useful in transfections of mammalian cell lines.
Main characteristics
| M | 238.31 |
|---|---|
| m.p | 52 - 58 °C |
Product specificities
| White to off-white powder | Complying |
| Iron (Fe) | <= 0.0005% |
| Loss on drying (105°C) | <= 0.5% |
| Potassium (K) | <= 0.1% |
| Heavy metals (as Pb) | <= 0.001% |
| pH (0.5M in water) | 5.0-6.5 |
| Sulfate (SO4) | <= 0.05% |
| Assay (T dry) | 99.5-101.0%w/w |
| UV Abs. 260nm (0.5M) | <= 0.05AU |
| UV Abs. 280nm (0.5M) | <= 0.05AU |
| DNase activity | Not detected |
| RNase activity | Not detected |
| Protease activity | Not detected |
