Dithiothreitol is an antioxidant, used in low concentrations to stabilise enzymes during protein purification and enzymatic reactions. At high concentration, Dithiothreitol reduces disulphide bridges present in polypeptides and facilitate protein denaturation by detergents or chaotropic agents.
| M | 154.24 |
|---|
| Appearance | White powder |
|---|---|
| Assay | 99-101%w/w |
| Heavy metals (as Pb) | max. 0.0005% |
| Oxidized DTT | max. 0.5% |
| A260nm (1%) | max. 0.5AU |
| A280nm (1%) | max. 0.1AU |
| Melting point | 39-44°C |
| Solubility (5% in Water) | Clear colorless |
| DNase activity | Not detected |
| RNase activity | Not detected |
| Protease activity | Not detected |
| Synonym : threo-1,4-Dimercapto-2,3-butanediol, Cleland's reagent, DTT |
| Formula : C4H10O2S2 |
| CAS : 3483-12-3 |
| EC : 222-468-7 |
| 04482349 | 6 x 10g | HDPE (10g) |
| 04482354 | 6 x 50g | Container (50g) |
| 04482356 | 1 x 100g | HDPE (100g) |
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