C8H18N2O4S
HEPES free acid Molecular biology
HEPES is a zwitterionic N-substituted acidic buffering substance used for culturing cells in vitro. HEPES is also useful in transfections of mammalian cell lines.
Main characteristics
Product specificities
Appearance | White to off-white powder |
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Iron (Fe) | max. 0.0005% |
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Loss on drying (105°C) | max. 0.5% |
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Potassium (K) | max. 0.1% |
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Heavy metals (as Pb) | max. 0.001% |
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pH (0.5M in water) | 5-6.5 |
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Sulfate (SO4) | max. 0.05% |
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Assay (T, dry) | 99.5-101.0%w/w |
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A260nm (0.5M) | max. 0.05AU |
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A280nm (0.5M) | max. 0.05AU |
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DNase activity | Not detected |
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RNase activity | Not detected |
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Protease activity | Not detected |
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