C21H20BrN3
Ethidium bromide Molecular biology
Ethidiumbromide is an aromatic cationic dye. Ethidiumbromide is commonly used for fluorometric detection of double stranded nucleic acids. Ethidiumbromide also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA’s.
Main characteristics
| M | 394.3 |
|---|---|
| m.p | 260-262 °C |
Product specificities
| Appearance | Burgundy powder |
|---|---|
| Assay (T) | min. 98%w/v |
| Loss on drying (105 | max. 5% |
| A525nm (1%/MeOH) | 145-165AU |
| Absorption max. (MeOH) | 524-527nm |
| DNase activity | Not detected |
| RNase activity | Not detected |
| Protease activity | Not detected |
