C6H13NO5

Tricine Molecular biology

Tricine is commonly used as a component of the Tris-Tricine-SDS (TTS) running buffer, with SDS-PAGE of proteins using the Shagger and von Jagow method. The method is designed for the separation of low molecular weight proteins and differs from the Laemmli method in that the Glycine is replaced with Tricine. This biological buffer has a usable pH range of 7.4-8.8.

Main characteristics

M179.17
m.p182-184 °C

Product specificities

AppearanceWhite powder
Solubility (10% in Water)Clear colorless solution
Loss on drying (105°C)max. 0.5%
Heavy metals (as Pb)max. 0.002%
pH (10% in Water)4.5-5.5
Assay (T)98.0-102.0%w/w
DNase activityNot detected
RNase activityNot detected
Protease activityNot detected

Cat. n° 203423

Synonym : N-[Tris(hydroxymethyl)methyl]glycine
Formula : C6H13NO5
CAS : 5704-04-1
EC : 227-193-6
<h5>C<sub>6</sub>H<sub>1</sub><sub>3</sub>NO<sub>5</sub></h5>

Standard pack

203423561 x 100gHDPE (100g)
203423576 x 250gHDPE (250g)
203423596 x 500gHDPE (500g)
203423916 x 1kgHDPE (1kg)

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